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POLYMER REACTIONS A DNA paper. Very Formal.
Title: POLYMER REACTIONS A DNA paper. Very Formal.
Category: Science & Technology / Biology
Details: Words: 5714 | Pages: 24.3 (approximately 235 words/page)
POLYMER REACTIONS A DNA paper. Very Formal.
Introduction
Polymerase chain reaction (PCR), can be used to amplify rare specific DNA sequences into many billions of molecules when the ends of the sequence are known. The method of amplifying rare sequences from a mixture has numerous applications in basic research, human genetics testing, and forensics. In one application, small samples of DNA, such as those found in a strand of hair at a crime scene, can produce sufficient copies to carry out forensic tests. In PCR Genomic DNA is digested into large fragments …showed first 85 words of 5714 total…
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…showed last 85 words of 5714 total…att1 and att2 sites, so that attL1 only reacts with attR1, and attL2 with attR2 (8).
The recombination reaction yields two constructs: the Expression Clone desired and a by-product, labeled here as the Donor Vector. The expression plasmid is under two forms of selection, antibiotic resistance and negative selection, supposedly ensuring a high level of cloning efficiency. The "reverse" or BP Reaction can also be done by recombining DNAs with attB and attP sites, respectively (8).
Excitation and Emission Spectra for
Living Colors® Fluorescent Proteins (5).



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