TRYPSIN LAB

Title: The Effects of Substrate Concentration and Temperature on the Rate of Hydrolysis of the Enzyme Trypsin. Abstract: Quantitative measurements can relate both temperature and substrate concentration to the enzymatic activity of trypsin. By analyzing the data, it is suggested that at BAPNA concentrations below those corresponding to Vmax are rate limiting, as less active sights are available for adhesion. The values of Vmax and Km relate a temperate catalytic efficiency of trypsin. The temperature …

…Being a biological enzyme, it would assume to work well at temperatures associated with biological life, which it did, working optimally within the range of 36-54 degrees Celsius. Below this temperature, little activity was observed as the molecules were moving in a slower fashion, and the shape once again is changed. References: Karp, G. (1996) Bioenergetics. Pages 91-103 in Karp, G., Cell and Molecular Biology: Concepts and Experiments Second Edition. John Wiley & Sons Inc., New York