Centrifugation Lab

INTRODUCTION The purpose of this experiment was to separate and identify the lower density particles in a homogenous mixture. Thus, the objective of the exercise was to realize that there is more than one structure in the cytoplasm and to isolate them using homogenization and differential centrifugation. In doing so, one can recognize and identify these particles by how the separate—which can be affected by density, shape, viscosity, the speed and/or diameter of …

…mitochondria to be present. Test tube C would not be expected to have mitochondrial activity because neither of the chemicals in test tube C came in contact with any of the pea particles. 3. If the experiment did not turn out as expected it would be because of human error or the fact that the chemicals used were tainted. 4. The results of this experiment illustrated the understanding of how structures separate by size, shape, and weight.