Diffusion and Osmosis

Principals and Practices of Diffusion and Osmosis A. Title: Diffusion and Dialysis Purpose: To determine permeability of a dialysis membrabe through the observation of different dyes’ ability to penetrate it. Hypothesis: Because molecules having molecular weights greater than 10,000 cannot penetrate the dialysis membrane, the orange dye, having molecular weight of 300, will penetrate the membrane while the violet dye, having a molecular weight of 100,000, will not and will continue to remain in tubing. Experimental Design: First, …

…would possibly decrease or remain the same. 6. In order to remove the salt from the protein solution, a diffusion and dialysis system would be set up in which the cut off value in molecular weight of the semipermeable membrane is less than 65,000 but large enough to allow the substance with the smaller molecular weight in the mixture to diffuse out of the membrane. This way the salt could, through dialysis, be separated from the mixture.