Dna gel electrophorosis

Introduction: DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic acid molecule which determines inherited structure of a protein. The “steps” are made of bases: adenine, guanine, cytosine, and thymine. The sides are sugar and phosphate molecules. Restriction enzymes are enzymes that cut DNA at restriction sites, leaving fragments blunt or sticky. The restriction fragments are separated using a technique called gel electrophoresis. DNA has a negative charge so when an electrical charge is applied …

…off after the bands migrated near the end of the gel and the top of the electrophoresis chamber was removed. We removed the gel from the gel casting tray and examined it under a light box and compared it to the ideal gel (figure1-2). Bibliography References: Restriction Enzymes: Cleavage of DNA lab University of Illinois. (1999). Experiment 2 Gel Electrophoresis of DNA. In Molecular Biology Cyberlab, online: Http://www.life.uluc.edu/molbio/geldigest/electro.html