Plasmid Fusion and PCR

This details the molecular biology lab in AP Biology. Best grade in class.         The AMGEN Lab that we have been doing for the past two weeks consisted of two parts; Plasmid Fusion and PCR. Each one is a complicated procedure of genetic engineering, with our own cheek cells and E.Coli supplied by AMGEN. I will start by explaining the Plasmid Fusion lab.         The Plasmid Fusion lab consisted of four major parts; plasmid digestion, gel …

…think that the fact that the wells were glowing means that the PCR did work and that the glowing product in the wells is the product of the PCR experiment. This is because in the PCR machine all the DNA is separated and jumbled, the primer helps to form the only full DNA structure so when we added the yellow loading buffer it could only bond with the full DNA which was the tPA-25-ALU.