What affect the substrate has on the rate of reaction with the enzyme in potato?

Enzyme Investigation Task To find out what affect the substrate has on the rate of reaction with the enzyme in potato. Variables 1.         PH 2.         Temperature 3.         Concentration of substrate 4.         Concentration of enzyme 5.         Surface area of potato lumps 6.         Time Key Variables Input variables - concentration of substrate Output variable - volume of gas Prediction I think that to start with, the higher the concentration of the substrate the faster the rate of reaction. However, I think that eventually …

…the temperature of the room in which the experiment is being done. I would also try and find a way to keep the size of my potato lumps more consistent so the variation in surface area does not affect my results. If I was going to do this experiment again I would also use some higher concentrations than where I stopped in this experiment because I could see the predicted curve of the line better.