creatinine

Experiment showing the determination of Creatinine concentration in 24-hour urine samples. Aim: The aim of the practical is to test the following hypothesis: “The amount of creatinine found in a persons 24-hour urine sample bears a direct relationship to the body mass of that person.” METHOD: Materials: 1) 5 urine samples of unknown concentration numbered 1, 5, 6, 7 ad 8. 2) Picric Acid 3) 0.75M Sodium Hydroxide 4) P1000 Gilson Pipette 5) 1cm3, 5cm3 and 10cm3 glass pipettes 6) Vortex Mixer 7) Spectrophotometer 8) Prafilm 9) Test Tubes Prior …

…same day so the effect of the weather will be the same on everyone. Experimental improvements include greater care when pipetting and more thorough calculations if student is unsure. Equipment should be high quality and should not under any circumstances be faulty. CONCLUSION: The hypothesis has been proven i.e. the creatinine concentration found in 24-hour sample of urine is directly related to the mass of a person. SABEEH SYED BSc BIOLOGICAL Sc. 1st YEAR