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Date Submitted: 10/26/2002 12:30:15
Length: 10 pages (2878 words)
Views: 187313

PHM499 Research Project Calcium transport study of SF-9 lepidopteran cells and bull frog sympathetic ganglion cells ABSTRACT The intracellular calcium level and the calcium efflux of the bull-frog sympathetic ganglion cells (BSG) and the SF-9 lepidopteran ovarian cells were investigated using a calcium-sensitive fluorescence probe fura-2. It was found that the intracellular calcium levels were 58.2 and 44.7 nM for the BSG cells and SF-9 cells respectively. The calcium effluxes following zero calcium solution were 2.02 and 1.33 fmole·…

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…-530; 1976. Reed, P. W.; Lardy, H. A. A23187: A divalent cation ionophore. J. Biol. Chem. 247:6970-7; 1972. Schwartz, J.-L.; Garneau, L.; Masson, L.; Brousseau, R. Early response of cultured lepidopteran cells to exposure to SYMBOL 100 f "GreekMathSymbols"-endotoxin from Bacillus thuringiensis: involvement of calcium and anionic channels. Biochem. Biophys. Acta 1065:250-260; 1991. Summers, M. D.; Smith, G. E. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agric. Exper. Sta. Bull. no 1555; 1987.

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