Electrophoresis Separation of Proteins Cytochrome C Myoglobin Hemoglobin and Serum Albumin by Using Isoelectric Focusing System IEF

Electrophoresis Separation of Proteins Cytochrome C, Myoglobin, Hemoglobin, and Serum Albumin by Using Isoelectric Focusing System (IEF) Introduction Proteins are composed of amino acids. All amino acids are amphoteric molecules consisting of three types of amino acids: neutral, acidic, and basic. Thus, for any protein there is a characteristic pH, called the isoelectric point (pI), at which the protein has no net charge and therefore will not move in the electric field. Electrophoresis takes advantage …

…Midgard, R., and Vedeler, CA. (2000 Nov). Oligoclonal bands in cerebrospinal fluid: a comparative study of isoelectric focusing, agarose gel electrophoresis and IgG index. [Abstract] Acta Neurol Scand 2000 Nov;102(5). 322-5. Retrieved February 2, 2001 from the WWW: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11083510&dopt=Abstract Natural Toxins Research Center at Texas A&M University. Isoelectric focusing. Retrieved January 29, 2001 from the WWW: http://ntri.tamuk.edu/if/if.html