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How various mechanisms by which substances cross the cell membrane
is hydrolyzed and the phosphate group is transferred we call it phosphorylation. In review, substances cross the cell membrane in two different ways: passive and active transport. Passive transport includes osmosis, diffusion, and facilitated diffusion. None of these techniques use energy. Active transport includes endo and exocytosis, sodium-potassium pumps. and phosphorylation, all of which expend energy to transport substances across the cell membrane. The cell has the ability to regulate what crosses its cellular boundaries.
