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TESTING THE FUNCTIONAL ROLE OF CHROMOSOME 13 IN THE TRANSFORMATION OF HUMAN BREAST EPITHELIAL CELLS in vitro Mathew Thomas, Grade 11 Recent empirical evidence suggests that genomic alterations play a crucial role in breast cancer development. The functionality of genomic alterations is demonstrated by utilizing micro-cell mediated chromosome transfection (MMCT) techniques. According to recent findings, the immortalization and tumorigenesis ability of human breast epithelial cells (HBEC) maybe associated with the microsatellite instability of the human chromosomes 9, 11, 13, and 17. …

…the transfection procedures for the cell lines and after the desired 21-day waiting period, has tabulated secondary colony sizes and efficiencies for the newly transfected cell lines. With these quantitative and qualitative data, it can be seen that there is indeed a very dramatic colony size decrease. With the hypothesis now supported, one can conclude that there is a definite possibility in developing a treatment for pre-existing breast cancer using the human chromosome 13. ------------------------------------------------------------------------ **Bibliography**