Investigation into the Use of Short-Column Reversed-Phase LC in the Determination of log P Values

Aim To investigate the possibility of determining log P via Reversed-Phase LC Introduction Increasingly, the determination of low concentrations of active ingredients in complex mixtures, sold for human consumption, has become more necessary. Regulations have imposed strict limits on the type and concentrations of a host of substances sold as foods or drugs. Such requirements demand analytical techniques that are fast and reliable and combine the separation and analysis steps in a single operation. Chromatography …

…has important uses from the point of view of the drug. Papp is related to P which is a ratio of drug dissolved in aqueous and organic phases. Papp differs from P as it takes into account the ionisation of the drug. This is important as most drugs when they change to acid or base they become ionised and their solubility's change. The Papp can then give us useful information for the compound in question