Using Electrophoresis To Determine The Sickle Cell Disease I

Title: Using Electrophoresis To Determine The Sickle Cell Disease In Hemoglobin Purpose: Electrophoresis is the movement of charged molecules under the influence of an electric field. Because amino acids and proteins are charged molecules, they migrate in an electric field at appropriate pH values. Hemoglobin is the major protein inside red blood cells. Its primary function is to transport oxygen. When used in an electrophoresis experiment traits and diseases are easily determined because of where …

…in this procedure. The experimenters could have placed different amounts of samples for electrophoresis in the wells of the agarose gel. This could have affected the visibility or concentration of the stains on the gel. There is always the possibility of contamination when working with several other groups and several types of samples. The pipettes may have been switched by accident, or perhaps some amount of one sample could have contaminated the pipette of another.