Biology A level planning experiment of effect of lead ions on amylase

The effect of Lead ions on amylase activity Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect, which causes the substrate, in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible, which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate …

…and by doing this denaturing the active site. References: *1 =http://www.courses.psu.edu/fd_sc/fd_sc400_jnc3/proteins/enzymes.htm *2 =http://userpages.wittenberg.edu/s04.bdoane *3 =http://www.lsbu.ac.uk/biology/enztech/inhibition.html *4 = http://www-saps.plantsci.cam.ac.uk/records/rec259.htm *5 =http://www.coursework.info/i/19208.html *6 =http://www.accessexcellence.org/AE/AEPC/WWC/1991/enzyme_substrate.html *7 =http://aem.asm.org/cgi/content/abstract/59/3/706 *8 =http://www.healthynewage.com/pure.html