CHROMATOGRAPHY

OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper, graduated cylinders, Erlenmeyer flask, mortar and pestle, metric ruler, tall …

…were already given, differed from the calculated lab values (weren't exactly the same as the given values), but in general, all five of the pigments were present in the plant leaf. All the data were recorded or shown in the chromatogram, a pattern formed by substances that have been separated by chromatography. The result is the chromatogram showing all five of the colored pigments in different places, in relation to the distance the solvent traveled.