Effects of Enzyme Rate by Variables of Enzyme Concentration, Substrate Concentraion, Temperature and pH

Abstract This study was undertaken to determine the effects of different environments affecting the rate of reaction, PNPP (p-nitrophenyl phosphate) + H20 → PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase, which alters its active site, and thus changes its efficiency to catalyze the reaction. We measured …

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