Gram Staining to Distinguish Between Different Types of Bacteria

It/'s a practical Good and to the point. Gram Staining to Distinguish Between Different Types of Bacteria Introduction Bacteria fall into two natural groups according to their cell wall structure. Some are stained with Gram's stain and are termed Gram positive, others do not retain the stain during the decolourising procedure and are termed Gram negative. Aim To test whether B. subtilis and A. tumefaciens are Gram positive or Gram negative using the Gram …

…saliva and other body fluids and egg white. Lysosome breaks the polysaccharide backbone of murein by catalysing hydrolysis of certain sugar linkages. The wall is thus punctured and lysis of the cell occurs if the organism is in a hypotonic solution. The same lipid-rich layer also confers resistance to penicillin, which attacks Gram positive bacteria by interfering with the cross-linking in growing cells, thus making the wall less rigid and more susceptible to osmotic shock.